Immunofixation Electrophoresis

In general, electrophoresis is a test method based on the separation of proteins with different electrical charges by applying electricity in a liquid or solid medium where the sample to be tested are placed., According to the strength of their electrical charges proteins move towards the opposite charged poles .This media is stained with specific protein dyes and the coloured proteins are measured optically according to the optical wavelengths of the dyes giving the information on the different proteins which are present in the sample being tested. This method is used in seperating and identifying many protein types like hemoglobin, blood proteins, enzymes, immunoglobulins and paraproteins ( ectopic proteins which do not belong to the original genetic code of that organism)
Interpretation:Immunglobulin test is done especially to detect a paraprotein which does not originate from the body’s own genetical code but originated from an ectopic source like a tumor. The body then tends to fight against this protein considering that it is a foreign substance. The antibody production against these non recognizable proteins is called monoclonal gammopathy and is mostly related to malign or benign tumors.
Sample: Arm venous blood. Nonfasting
Working day: Everyday
Result Time: Three days later, at 6 PM